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Updated: Jun 28, 2026

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
04:41

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

[Apoptosis of human keloid fibroblast induced by small interfering RNA-mediated CyclinD1 gene silencing]

Da-Ning Liang1, Jian-Hua Gao, Feng Lu

  • 1Department of Plastic Surgery, Nanfang Hospital of the Southern Medical University, Guangzhou 510515, China.

Zhonghua Zheng Xing Wai Ke Za Zhi = Zhonghua Zhengxing Waike Zazhi = Chinese Journal of Plastic Surgery
|October 28, 2008
PubMed
Abstract

Insights

Small interfering RNA targeting CyclinD1 (siRNA-CyclinD1) effectively inhibits CyclinD1 gene expression in human keloid fibroblasts. This inhibition leads to cell cycle arrest at the G1 stage and increased apoptosis, suggesting potential RNAi therapy for keloids.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Dermatology

Context:

  • Keloids are characterized by excessive fibroblast proliferation.
  • CyclinD1 is a key regulator of cell cycle progression.
  • Targeting CyclinD1 offers a potential therapeutic strategy for keloid treatment.

Purpose:

  • To investigate the inhibitory effect of CyclinD1-specific small interfering RNA (siRNA-CyclinD1) on CyclinD1 gene expression in human keloid fibroblasts.
  • To evaluate the impact of siRNA-CyclinD1 on keloid fibroblast cell cycle, proliferation, and apoptosis.

Summary:

  • siRNA-CyclinD1 was designed and transfected into human keloid fibroblasts.
  • RT-PCR confirmed significant downregulation of CyclinD1 mRNA expression.
  • Flow cytometry revealed G1 cell cycle arrest and increased apoptotic rates, with DNA fragmentation observed.

Impact:

  • siRNA-CyclinD1 effectively inhibits CyclinD1 expression in keloid fibroblasts.
  • This inhibition arrests the cell cycle at G1 and enhances apoptosis.
  • The findings provide preliminary evidence for RNAi-based therapy for keloids.

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