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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

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Published on: January 27, 2013

Screening blockers against a potassium channel with a droplet interface bilayer array.

Ruhma Syeda1, Matthew A Holden, William L Hwang

  • 1Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK.

Journal of the American Chemical Society
|October 28, 2008
PubMed
Summary

Droplet interface bilayers enable spontaneous insertion of membrane proteins like ion channels. This allows for a new chip-based method to rapidly screen potential channel blockers.

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Last Updated: Jun 28, 2026

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
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Published on: January 27, 2013

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Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
10:41

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

Published on: March 7, 2018

Area of Science:

  • Biophysics
  • Membrane protein research
  • Biotechnology

Background:

  • Droplet interface bilayers (DIBs) are a model system for studying membrane proteins.
  • Both alpha-helix bundle and beta-barrel proteins spontaneously insert into DIBs.
  • Cell-free expression systems allow for rapid protein production.

Purpose of the Study:

  • To develop a chip-based platform for high-throughput screening of ion channel blockers.
  • To demonstrate the utility of DIBs for functional studies of membrane proteins.

Main Methods:

  • Formation of DIBs from lipid monolayer-coated aqueous droplets in oil.
  • Spontaneous insertion of cell-free expressed ion channels (Kcv) into DIBs.
  • Electrode incorporation for measuring ion channel activity and screening blockers.

Main Results:

  • Demonstrated spontaneous insertion of both channels and pores into DIBs.
  • Successfully measured ion currents through individual Kcv channels.
  • Validated the chip-based approach for rapid screening of channel blockers.

Conclusions:

  • DIBs provide a versatile platform for studying membrane protein function.
  • The developed chip-based method enables efficient screening of ion channel modulators.
  • This approach has potential applications in drug discovery and fundamental membrane protein research.