Related Experiment Video
Updated: Jun 28, 2026
08:35
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
Determining the impact of alternative splicing events on transcriptome dynamics
Emmanuelle Wilhelm1, François-Xavier Pellay, Arndt Benecke
1RNA Group, Département de microbiologie et d'infectiologie, Faculté de médecine et sciences de la santé, Université de Sherbrooke, 3001 12e ave Nord, Sherbrooke, Québec J1H 5N4, Canada. Emmuanuelle.Willhelm@USherbrooke.ca
BMC Research Notes
|October 28, 2008
Summary
This study introduces a method to track gene expression changes caused by alternative splicing. Researchers found that Bcl-xS isoform expression represses genes involved in protein synthesis.
Area of Science:
- Molecular Biology
- Genomics
- Gene Expression Analysis
Background:
- Alternative splicing significantly contributes to proteome diversity.
- Splice switching oligonucleotides (SSOs) are tools to control alternative splicing and have therapeutic potential for splicing-related diseases.
- Understanding the function of unknown alternative splicing events can reveal new therapeutic targets.
Purpose of the Study:
- To develop a protocol for monitoring global gene expression changes downstream of alternative splicing events.
- To investigate the biological impact of specific alternative splicing events on gene expression.
Main Methods:
- Combining high-sensitivity microarrays with SSO transfection.
- Monitoring global gene expression changes in response to controlled alternative splicing.
Main Results:
- The protocol successfully linked the induction of the Bcl-xS isoform to the repression of protein synthesis genes.
- Demonstrated a connection between Bcl-xS expression and the downregulation of ribosomal protein gene expression.
Conclusions:
- The developed strategy effectively defines the biological impact of alternative splicing events on global gene expression.
- Established the first link between Bcl-xS expression and the repression of ribosomal protein gene expression, highlighting a novel regulatory mechanism.
Related Concept Videos
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:41Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
01:36What is Gene Expression?
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...