Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complex interactions between polyamines and calpain-mediated proteolysis in rat brain.

I Najm1, P Vanderklish, A Etebari

  • 1Section of Neurobiology, University of Southern California, Los Angeles 90089-2520.

Journal of Neurochemistry
|October 1, 1991
PubMed
Summary

Polyamines, like spermine and spermidine, enhance calcium-dependent protein breakdown by calpains in rat brain. This suggests polyamines play a role in both protein synthesis and degradation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurements of branching fractions and time-dependent CP-violating asymmetries in B --> eta'K decays.

Physical review letters·2005
Same author

Search for decays of B0-->e+ e-, B0-->mu+ mu-, B0-->e +/- mu-/+.

Physical review letters·2005
Same author

Branching fraction and CP asymmetries of B0-->K0(S)K0(S)K0(S).

Physical review letters·2005
Same author

Search for lepton flavor violation in the decay tau+/- --> mu+ gamma.

Physical review letters·2005
Same author

Search for the rare leptonic decay B--->tau-nutau.

Physical review letters·2005
Same author

Limit on the B0-->rho0rho0 branching fraction and implications for the Cabibbo-Kobayashi-Maskawa angle alpha.

Physical review letters·2005

Area of Science:

  • Neurochemistry
  • Molecular Biology
  • Biochemistry

Background:

  • Polyamine synthesis is triggered by extracellular signals and linked to cell growth and differentiation.
  • Brain tissue stimulation increases protein breakdown via calcium-dependent proteases (calpains).

Purpose of the Study:

  • To investigate the potential modulation of calpain activity by polyamines.

Main Methods:

  • Assessed the effect of spermine, spermidine, and putrescine on calcium-dependent proteolysis of casein and endogenous proteins in rat brain fractions.
  • Utilized [14C]casein and spectrin as substrates for purified calpain I and II.
  • Examined the influence of polyamines on calpain activity in the presence of activators and inhibitors like leupeptin and calpastatin.

Related Experiment Videos

Main Results:

  • Polyamines (spermine > spermidine > putrescine) increased calcium-dependent proteolysis of casein and endogenous structural proteins in rat brain extracts.
  • Polyamines potentiated the activation of endogenous calpain activity but not purified calpain.
  • Leupeptin inhibited proteolysis, while polyamines did not affect calpastatin inhibition.

Conclusions:

  • Polyamines modulate calpain activity, suggesting a role in regulating protein degradation in the brain.
  • The findings indicate a dual role for polyamines in both protein synthesis and degradation processes.