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Identification of B2 bradykinin binding sites on cultured cortical astrocytes
A J Cholewinski1, G Stevens, A M McDermott
1Biochemistry Department, Imperial College of Science, Technology and Medicine, London, England, U.K.
Journal of Neurochemistry
|October 1, 1991
Abstract:
Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single KD of 16.6 +/- 2.6 nM and a Bmax of 352.2 +/- 30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.