Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of Protein Sumoylation.

Current protocols in protein science·2016
Same author

Heat shock transcription factor 1 is activated as a consequence of lymphocyte activation and regulates a major proteostasis network in T cells critical for cell division during stress.

Journal of immunology (Baltimore, Md. : 1950)·2013
Same author

WITHDRAWN: Protein sumoylation and human diseases.

Biochimie·2012
Same author

VDR and CYP27B1 are expressed in C2C12 cells and regenerating skeletal muscle: potential role in suppression of myoblast proliferation.

American journal of physiology. Cell physiology·2012
Same author

SUMO and its role in human diseases.

International review of cell and molecular biology·2011
Same author

PEST sequences mediate heat shock factor 2 turnover by interacting with the Cul3 subunit of the Cul3-RING ubiquitin ligase.

Cell stress & chaperones·2009

Related Experiment Video

Updated: Jun 28, 2026

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

Detection of sumoylated proteins.

Ok-Kyong Park-Sarge1, Kevin D Sarge

  • 1Department of Physiology, University of Kentucky, Lexington, KY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2008
PubMed
Summary

This study details methods to identify protein sumoylation targets and specific modification sites. Understanding small ubiquitin-related modifier (SUMO) conjugation is key to cellular function and nuclear processes.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Small ubiquitin-related modifier (SUMO) is an ubiquitin-like protein involved in post-translational modification.
  • Unlike ubiquitination, sumoylation regulates protein function, not degradation.
  • Sumoylation is crucial for nuclear processes and structure.

Purpose of the Study:

  • To present methodologies for identifying novel SUMOylation substrates.
  • To determine the specific lysine residues targeted by SUMOylation.
  • To advance the understanding of SUMOylation's role in regulating protein function.

Main Methods:

  • Experimental protocols for assessing protein sumoylation.
  • Techniques for pinpointing SUMOylation sites on target proteins.

More Related Videos

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
10:12

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

Related Experiment Videos

Last Updated: Jun 28, 2026

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
10:12

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

  • Biochemical assays to validate substrate and site identification.

    • Main Results:

    • Established methods for confirming protein sumoylation.
    • Identified specific lysine residues as sites of SUMOylation.
    • Provided a framework for discovering new SUMOylation targets.

    Conclusions:

    • Characterization of sumoylation is vital for understanding nuclear function.
    • The presented methodologies facilitate the identification of SUMOylation substrates and sites.
    • This work aids in elucidating the regulatory roles of SUMOylation in cellular processes.