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Updated: Jun 28, 2026

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
Published on: July 14, 2015
Inferring molecular function: contributions from functional linkages
Arturo Medrano-Soto1, Debnath Pal, David Eisenberg
1Howard Hughes Medical Institute (HHMI), 675C. E. Young Drive South, Los Angeles, CA 90095, USA.
Abstract:
In the current era of high-throughput sequencing and structure determination, functional annotation has become a bottleneck in biomedical science. Here, we show that automated inference of molecular function using functional linkages among genes increases the accuracy of functional assignments by > or =8% and enriches functional descriptions in > or =34% of top assignments. Furthermore, biochemical literature supports >80% of automated inferences for previously unannotated proteins. These results emphasize the benefit of incorporating functional linkages in protein annotation.
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