Related Experiment Video
Updated: Jun 28, 2026

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
Published on: May 12, 2023
A bulge binding agent with novel wedge-shape topology for stimulation of DNA triplet repeat strand slippage synthesis
Liangliang Liu1, Heyang Li, Long Yi
1State Key Laboratory of Elemento-Organic Chemistry, Department of Chemical Biology, Nankai University, 94 Weijin Road, Tianjin 300071, China.
Abstract:
Expansion of DNA repeat sequences is associated with many human genetic diseases. Bulged DNA structures have been implicated as intermediates in DNA slippage within the DNA repeat regions. Herein a bulge binding agent with novel wedge-shape topology of the aromatic moiety was designed and synthesized. The compound-bulge DNA interactions were characterized via UV melting experiments, circular dichroism and were quantitated by surface plasmon resonance with K(d) of 41.5 microM. This compound showed remarkable stimulation for DNA triplet repeat strand slippage synthesis in vitro.
Related Concept Videos
Single-Strand DNA Binding Proteins
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
The DNA Replication Fork
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair

