Expression of virus specific morphological cell transformation induced in enucleated cells

Chick embryo fibroblasts infected by a temperature sensitive mutant of Rous sarcoma virus were enucleated with Cytochalasin B. The cytoplasts were still able to transform morphologically when shifted from nonpermissive to permissive temperature, and to revert ot normal morphology when shifted from permissive to nonpermissive temperature. This indicates that the viral gene product responsible for transformation acts primarily on cytoplasmic or membrane components and not on the nucleus.