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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

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Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen
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Hepatic gene expression during endotoxemia.

Roland S Croner1, Werner Hohenberger, Marc G Jeschke

  • 1Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany. Roland.Croner@uk-erlangen.de

The Journal of Surgical Research
|October 28, 2008
PubMed
Summary
This summary is machine-generated.

Sepsis alters liver function by shifting gene expression from metabolism to immune response, causing hepatic damage. Microarray analysis revealed significant gene regulation changes, highlighting the liver's immune role in sepsis.

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Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Area of Science:

  • Hepatology
  • Immunology
  • Molecular Biology

Background:

  • Sepsis involves endotoxins and cytokines activating Kupffer cells, leading to liver damage.
  • The complex interactions between hepatic cells in septic liver failure are not fully understood.
  • Microarray technology offers a potential tool to elucidate these cellular interactions.

Purpose of the Study:

  • To investigate the gene expression changes in the liver during sepsis using microarray analysis.
  • To understand the molecular mechanisms underlying septic liver failure.
  • To identify key genes and pathways involved in the hepatic response to sepsis.

Main Methods:

  • Rats were injected with lipopolysaccharides (LPS) or saline.
  • Liver tissue was collected 24 hours post-injection for RNA isolation.
  • Microarray analysis (RAE 230A) was performed, with key gene expressions validated by RT-PCR and immunohistochemistry.

Main Results:

  • 508 differentially expressed genes were identified between LPS and saline groups.
  • Genes related to immune response and receptor activity were predominantly upregulated post-LPS.
  • Genes involved in metabolism and catalytic activity were downregulated in the LPS group.
  • Microarray findings were confirmed by RT-PCR and immunohistochemical staining.

Conclusions:

  • Sepsis induces a significant shift in liver gene expression from metabolic to immune functions.
  • This metabolic to immunological switch exacerbates hepatic damage during sepsis.
  • Further research is needed to clarify the functional interactions of identified genes in sepsis-induced liver injury.