Related Experiment Video
Updated: Jun 28, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Analysis of hepatitis B virus X gene phylogeny, genetic variability and its impact on pathogenesis: implications in
Sibnarayan Datta1, Arup Banerjee, Partha Kumar Chandra
1ICMR Virus Unit, Kolkata, ID and BG Hospital Campus, GB-4, 1st Floor (East Wing), 57, Dr. Suresh Chandra Banerjee Road, Pin-700010 (Beliaghata), Kolkata, West Bengal, India.
Abstract:
HBx genetic variability was explored in the Eastern Indian population with low HCC incidence. DNase I sensitive HBV DNA was detected in 53% samples, which differed significantly between clinical groups (P<0.001). HBV genotypes A (Aa/A1), C (Cs/C1) and D (D1, D2, D3, D5) were detected in 37.5%, 18.7% and 43.7% samples respectively. Population specific signature HBx residues A(36), V(88), S(101) in Aa/A1 and residues P(41), Q(110) in D5 were detected. Mutations T(127), M(130) and I(131) were detected in 66.7%, 91% and 75% of genotype A, C and D5 samples respectively. Very low occurrence of HCC associated mutations (V(5)M/L, P(38)S, and H(94)Y) and absence of C-terminal deletions were observed. Our study shows that HBV genotype associated clinically important HBx variations may evolve and act distinctly in different geo-ethnic populations. Further studies on HBx functions from the perspective of genetic variability are essential for the better understanding of the clinical significance of HBV.
Related Concept Videos
Hepatitis
Viral Hepatitis I: Introduction
Viral Mutations
Retroviruses
Single Nucleotide Polymorphisms-SNPs
Human Virome

