Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Achieving a balance between mobilization and immobilization after surgical or conservative treatment of the hand.

The Journal of hand surgery, European volume·2026
Same author

Single versus repeated intravenous oncolytic reovirus infusions: Implications for immune modulation and rationalised scheduling of therapy in hepatocellular carcinoma.

International journal of cancer·2026
Same author

"We Realised We Were Not in it by Ourselves." Exploring Experiences of a Multidisciplinary Psychoeducational Workshop for Parents With a Child Diagnosed With Autism Spectrum Condition: A Thematic Analysis.

Clinical child psychology and psychiatry·2026
Same author

Promoting innovation in children's nurse education: the Welcome to Our World project.

Nursing children and young people·2025
Same author

A vaccine emergency-when to overrule parental refusal of vaccination at birth for prevention of vertical transmission of hepatitis B virus?

Archives of disease in childhood·2025
Same author

Drivers of Seclusion and Physical Restraint in an Acute Mental Health Unit: A Feature Analysis.

Issues in mental health nursing·2025

Related Experiment Video

Updated: Jun 28, 2026

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

IL-10 suppresses CD2-mediated T cell activation via SHP-1.

Alison Taylor1, Johan Verhagen, Tunç Akkoç

  • 1Swiss Institute of Allergy and Asthma Research (SIAF), Davos, Switzerland. at504@cam.ac.uk

Molecular Immunology
|October 28, 2008
PubMed
Summary

Interleukin-10 (IL-10) directly suppresses T cell activation by inhibiting CD2 signaling through the phosphatase SHP-1. This discovery reveals a new mechanism for IL-10

More Related Videos

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
14:01

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Published on: July 22, 2011

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)
14:15

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

Published on: June 18, 2012

Related Experiment Videos

Last Updated: Jun 28, 2026

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
14:01

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Published on: July 22, 2011

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)
14:15

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

Published on: June 18, 2012

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Signaling

Background:

  • Interleukin-10 (IL-10) is a critical immunosuppressive cytokine involved in maintaining peripheral tolerance.
  • The precise molecular mechanisms by which IL-10 directly suppresses T cell function remain incompletely elucidated.

Purpose of the Study:

  • To investigate the direct molecular mechanisms of T cell suppression mediated by IL-10.
  • To determine if IL-10 directly impacts T cell receptor (TCR) or co-stimulatory molecule signaling pathways.

Main Methods:

  • Utilized T cell cultures with and without endogenous IL-10 sources.
  • Investigated the role of src-homology-2 domain containing tyrosine phosphatase (SHP-1) using dominant-negative constructs and small inhibitory RNA (siRNA).
  • Employed SHP-1-deficient and IL-10-deficient mouse models for in vivo validation.

Main Results:

  • IL-10 directly inhibits CD2-mediated T cell activation and proliferation.
  • SHP-1 is essential for IL-10's suppressive effect on CD2 signaling.
  • CD2-induced T cell proliferation was suppressed by IL-10 in IL-10-deficient but not SHP-1-deficient T cells.

Conclusions:

  • IL-10 directly suppresses T cell activation by targeting CD2 co-stimulation.
  • SHP-1 acts as the key mediator in IL-10's direct suppression of CD2 signaling.
  • This identifies a novel pathway for IL-10-mediated T cell immune regulation.