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Updated: Jun 28, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

High content cell screening in a microfluidic device.

Raymond Cheong1, Chiaochun Joanne Wang, Andre Levchenko

  • 1Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA.

Molecular & Cellular Proteomics : MCP
|October 28, 2008
PubMed
Summary
This summary is machine-generated.

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This study introduces a microfluidic device for high-throughput, single-cell analysis of cell signaling networks. The device enables quantitative interrogation of signaling species, revealing insights into cellular responses to various stimuli.

Area of Science:

  • Cellular and Molecular Biology
  • Systems Biology
  • Biotechnology

Background:

  • Understanding complex cell signaling networks requires high-throughput methods to analyze multiple signaling species at the single-cell level.
  • Existing methods often lack the throughput or resolution for comprehensive analysis of dynamic cellular responses.

Purpose of the Study:

  • To develop and validate a microfluidic device for quantitative, high-throughput, single-cell analysis of cell signaling networks.
  • To enable detailed interrogation of signaling dynamics in response to diverse stimulation protocols.

Main Methods:

  • Development of a novel microfluidic device for single-cell analysis.
  • Utilizing immunofluorescence-based readouts for quantitative measurements.
  • Applying the device to assay kinases, transcription factors, and target genes.

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Last Updated: Jun 28, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

Main Results:

  • The device enables high-throughput, high-content measurement of signaling activities in thousands of individual cells.
  • Demonstrated quantitative interrogation of time-course signaling responses to soluble stimuli, inhibitors, and complex temporal patterns.
  • Revealed that NF-kappaB activity dynamics in individual cells mirror population averages, contrasting with previous findings.

Conclusions:

  • The microfluidic device provides a powerful platform for dissecting cell signaling networks at the single-cell level.
  • Enables high-resolution investigation of cellular heterogeneity and population-level dynamics.
  • Offers new avenues for understanding signaling mechanisms and drug responses.