Related Experiment Video
Updated: Jun 28, 2026

Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization
Published on: May 18, 2018
Influence of body carbohydrate store modification on catecholamine and lactate responses to graded exercise in
T Mikulski1, A Ziemba, K Nazar
1Department of Applied Physiology, Medical Research Center, Polish Academy of Sciences, Pawinskiego 5, 02-106 Warsaw, Poland. tomik@cmdik.pan.pl
Abstract:
Aim of this study was to elucidate the influence of carbohydrate store modification on exercise capacity and catecholamine thresholds. Nine sedentary students and 10 endurance athletes volunteered for the study consisting of four sessions in one-week intervals. During control session (C) subjects performed graded exercise till exhaustion with workload increasing by 50 Watts every 3 min after an overnight fast. Blood lactate and catecholamines were determined at rest, after each workload and at exhaustion. In the evening preceding each of the remaining three sessions subjects performed glycogen reducing exercise lasting 1.5 hrs at 70% HRmax. Till next morning they did not eat any meal but drank water. Two hours before exercise subjects were given either a high-carbohydrate (H-CHO) or a low-carbohydrate (L-CHO) meal of approx. 1000 kcal or remained fasted (F). Depletion of carbohydrate stores enhanced contribution of fat-derived energy substrates at submaximal workloads, but did not influence either maximal oxygen consumption, workload and lactate concentration or lactate threshold. Low carbohydrate availability resulted in elevated concentration of catecholamines only in untrained subjects. Ingestion of a meal either high or low in carbohydrates diminished those changes. Catecholamine thresholds were similar in all sessions and higher in athletes' group.
Related Concept Videos
Exercise and Cardiovascular Response
Light to moderate physical activity initiates a series of interconnected responses in the body. The heart rate modestly increases in anticipation of the workout, followed by widespread vasodilation as oxygen consumption by skeletal muscles increases. This results in decreased peripheral resistance, increased capillary blood flow, and accelerated...
Glucose Homeostasis: Regulation of Blood Glucose
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
Overview of Carbohydrate Metabolism
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
Exercise and Muscle Performance
Endurance exercises
Endurance exercises involve running, swimming, or cycling, which require repetitive movements with low force output. When a person engages in endurance exercise, a few noticeable changes occur in their skeletal muscles. For instance, the number of capillaries...
Muscle Recovery and Fatigue
Exercise and Cardiac Output
Sustained exercise increases the muscles' oxygen demand, which can be met...
