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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Organization of Genes02:07

Organization of Genes

Overview

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Related Experiment Video

Updated: Jun 28, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Structural basis for exon recognition by a group II intron.

Navtej Toor1, Kanagalaghatta Rajashankar, Kevin S Keating

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06520, USA.

Nature Structural & Molecular Biology
|October 28, 2008
PubMed
Summary
This summary is machine-generated.

Group II introns are mobile genetic elements that splice into RNA and DNA. We determined the crystal structure of a group II intron complexed with its target RNA, revealing key structures for exon recognition.

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Related Experiment Videos

Last Updated: Jun 28, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Genetics

Background:

  • Group II introns are retroelements capable of reverse splicing into RNA and DNA.
  • Understanding their mechanism is crucial for studying mobile genetic elements and gene regulation.

Purpose of the Study:

  • To elucidate the structural basis of group II intron target recognition and binding.
  • To provide atomic-level insights into the reverse splicing mechanism.

Main Methods:

  • X-ray crystallography was used to determine the structure of the group II intron-oligonucleotide complex at 3.4-A resolution.
  • Refinement of the free group II intron structure was also performed.

Main Results:

  • The crystal structure of the complex reveals the precise conformation of the group II intron bound to its oligonucleotide target.
  • Specific motifs involved in exon recognition by the intron are visualized in the complex structure.
  • The structure of the free intron was refined, providing a baseline for conformational changes upon binding.

Conclusions:

  • The determined structure provides a detailed molecular understanding of how group II introns recognize and bind their target RNA substrates.
  • These findings offer insights into the mechanism of reverse splicing and the mobility of these retroelements.