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Updated: Jun 28, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Prevalence of hepatitis B virus genotypes in chronic carriers in Santiago, Chile
Mauricio Venegas1, Gabriela Muñoz, Carmen Hurtado
1Sección de Gastroenterología, Departamento de Medicina, Hospital Clínico de la Universidad de Chile, Av. Santos Dumont 999, Independencia, Santiago, Chile. mvenegas@redclinicauchile.cl
Abstract:
The eight genotypes (designated A-H) of hepatitis B virus (HBV) display distinctive geographical distribution worldwide, with genotypes A, D and F frequently detected in South America. To determine the prevalence of HBV genotypes in Santiago, Chile, 131 samples from chronic carriers were used for PCR amplification, and genotyping was performed by RFLP. The results indicated that genotype F was the most prevalent among HBV carries (84% of the cases), whereas genotypes A, B, C and D were found at a prevalence of 3.8, 3.8, 6.1, and 2.3%, respectively. We discuss these data in the complex scenario of HBV epidemiology.
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