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Second-order data obtained by time-resolved room temperature phosphorescence. A new approach for PARAFAC
A Muñoz de la Peña1, N Mora Diez, D Bohoyo Gil
1Department of Analytical Chemistry, University of Extremadura, 06071, Badajoz, Spain. arsenio@unex.es
Journal of Fluorescence
|October 28, 2008
Summary
This study introduces a new method combining PARAFAC and room temperature phosphorescence (RTP) to analyze mixtures. This approach simplifies analysis by not requiring deoxygenation and successfully resolves phenanthrene and 1,10-phenanthroline.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Chemometrics
Background:
- Room temperature phosphorescence (RTP) offers a sensitive detection method.
- Multivariate calibration is essential for analyzing complex mixtures.
- Resolving mixtures of similar compounds like phenanthrene and 1,10-phenanthroline presents analytical challenges.
Purpose of the Study:
- To develop and validate a second-order multivariate calibration method for analyzing binary mixtures.
- To utilize time-resolved room temperature phosphorescence (RTP) for enhanced analytical performance.
- To resolve a model mixture of Phenanthrene and 1,10-Phenanthroline.
Main Methods:
- Application of a second-order multivariate calibration approach.
- Combination of the Parallel Factor Analysis (PARAFAC) algorithm with time-resolved RTP.
- Acquisition of RTP signals in aqueous beta-cyclodextrin solutions with heavy atom compounds.
Main Results:
- Successful resolution of a binary mixture of Phenanthrene and 1,10-Phenanthroline.
- Demonstration that deoxygenation is not required for phosphorescence signal acquisition, simplifying the method.
- Utilization of differences in RTP signal delay-times for analyte resolution.
Conclusions:
- The combined PARAFAC-RTP method provides an effective approach for second-order data generation and multivariate calibration.
- This method offers a simplified and robust way to analyze mixtures without deoxygenation.
- The technique shows potential for analyzing complex samples in various fields.
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