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Updated: Jun 28, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
First description of Grapevine leafroll-associated virus 5 in Argentina and partial genome sequence
Sebastián Gómez Talquenca1, Claudio Muñoz, Oscar Grau
1Plant Virology Laboratory, EEA Mendoza INTA, San Martín 3853, (5507) Luján de Cuyo, Mendoza, Argentina. gtalquenca@mendoza.inta.gov.ar
Abstract:
An accession of Vitis vinifera cv. Red Globe from Argentina, was found to be infected with Grapevine leafroll-associated virus-5 by ELISA. It was partially sequenced, and three ORFs, corresponding to HSP70h, HSP90h, and CP, were found. This isolate shares a high aminoacid identity with the previously reported sequence of the virus, and identities between 80% and 90% with previously reported GLRaV-9 and GLRaV-4 isolates. The analysis of the sequence supports the clustering together with GLRaV-4 and GLRV-9 inside the Ampelovirus genus.
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