Related Experiment Video
Updated: Jun 28, 2026

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
[Application of molecular method in identification of bacterial endophthalmitis]
Wei Wu1, Xin-xin Lu, Yan-fei Huang
1Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital University of Medical Sciences, Beijing 100730, China.
Objective:
To search for a non-culture method to improve the efficiency of etiological diagnosis of infective endophthalmitis and other infectious diseases.
Methods:
Fifty clinical specimens of infective endophthalmitis were collected. DNA of bacterium was extracted directly from the aqueous fluid or vitreous fluid and then amplified with universal primers. The PCR products were sequenced subsequently.
Results:
Twenty-two (44%) bacterial smears and 13 (26%) cultured specimens were positive. PCR positivity was seen in 28 of the 50 cases (56%) and 22 specimens (44%) were sequenced successfully. The results of 16s rRNA gene sequence analysis did not completely conform to the results obtained from culture-dependent approach.
Conclusion:
Molecular identification method is a rapid and efficient approach for the diagnosis of bacterial infective endophthalmitis.
Related Concept Videos
Methods of Classification and Identification
Applications of Molecular Taxonomy
Rapid Identification of Pathogens
Modern Molecular Taxonomy
Automated Microbial Diagnostics
Methods to Assess Microbial Populations

