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Updated: Jun 28, 2026

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
Published on: November 22, 2019
[Differential transcription of Bcl-2 and Bax through the cell cycle in prostate cancer cell line]
Xue-Song Li1, Kan Gong, Guo-Feng Sun
1Department of Urology, Institute of Urology, Peking University First Hospital, Beijing 100034, China.
Objective:
To investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.
Methods:
The prostate cancer cell line PC-3 was synchronized in M, G1, S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique. The efficiency of synchronization was detected by flow-cytometry. RT-PCR and Western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.
Results:
The synchronized rate of M, G1, S and G2 phase were 92.1%, 87.0%, 80.2% and 75.9% respectively. Bcl-2 was constitutively expressed through the cell cycle, but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase, dramatically decreased in M, S and G2 phase. The expression level of Bax had no change through the cell cycle.
Conclusions:
Cell cycle could influence the expression level of Bcl-2 significantly but not Bax, these might have some clinical relevance.
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