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[Construction and identification of small interfering RNA expression plasmid targeting Sox9 and the function to cell
Hong-Min Qin1, Hui-Feng Han, Tie Xu
1Xuzhou Medical College Hospital, Xuzhou 221002, China. qinhongmin07@yahoo.com.cn
Objectives:
To construct small interfering (siRNA) Sox9 expression plasmid and transfer it into human chondrosarcoma cells HTB-94, and to check the mRNA and protein expression of Sox9 and cell growth and apoptosis of HTB-94 human chondrosarcoma cells.
Methods:
siRNA(Sox9) expression plasmid was designed and synthesized. And it was transferred into HTB-94 human chondrosarcoma cells. Then the expression of the mRNA and protein of Sox9, cell growth and apoptosis in transferred HTB-94 human chondrosarcoma cells were checked.
Results:
The recombinant plasmid was confirmed by enzyme digestion analysis and DNA sequencing. The expression of the mRNA and protein expression of Sox9 in transferred HTB-94 were significantly reduced. The cell growth of HTB-94 was inhibited, and the apoptosis of HTB-94 was remarkably increased.
Conclusion:
siRNA (Sox9) expression plasmid could be transferred into HTB-94 human chondrosarcoma cells. And it can reduce the mRNA and protein expression of the HTB-94, inhibit the cell growth and cause the apoptosis of the tumor cells.
Insights
Small interfering RNA (siRNA) targeting Sox9 was successfully delivered into human chondrosarcoma cells, significantly reducing Sox9 expression and inhibiting tumor cell growth while promoting apoptosis.
Area of Science:
- Molecular Biology
- Cancer Research
Context:
- Chondrosarcoma is a rare bone cancer.
- Sox9 is a transcription factor implicated in chondrosarcoma development.
Purpose:
- To construct and deliver a small interfering RNA (siRNA) Sox9 expression plasmid into human chondrosarcoma cells (HTB-94).
- To evaluate the impact of Sox9 knockdown on cell growth and apoptosis in HTB-94 cells.
Summary:
- A synthesized siRNA Sox9 expression plasmid was successfully transfected into HTB-94 cells.
- Enzyme digestion and DNA sequencing confirmed the recombinant plasmid.
- Transfection significantly reduced both mRNA and protein expression of Sox9.
- HTB-94 cell proliferation was inhibited, and apoptosis was markedly increased.
Impact:
- This study demonstrates the efficacy of siRNA-mediated Sox9 knockdown in inhibiting chondrosarcoma cell growth.
- The findings suggest a potential therapeutic strategy targeting Sox9 in chondrosarcoma treatment.
