Related Experiment Video
Updated: Jun 28, 2026

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
Published on: October 13, 2020
Abstract:
The substantiation and definition of the term "fragmentomics" are given. Within the framework of this scientific direction, a theoretical structural and functional analysis of all possible fragments of one protein molecule was performed to determine the sites that could be potential sources of the regulatory oligopeptides. For this purpose, the data on the primary structure of bovine hemoglobin, the information of the database EROP-Moscow on the structure and functions of natural oligopeptides, and a special computer program complex were used. As a result, natural nonhemoglobin oligopeptides containing hemoglobin fragments and natural oligopeptides representing the exact structures of hemoglobin fragments were found. Most abundant among them are neuropeptides, antimicrobial oligopeptides, and hormones. It has been shown that bovine tetrapeptide fragments identified in nonhemoglobin oligopeptides possessing the given functions occur in the amino acid sequences of experimentally obtained hemoglobin oligopeptides having the same functions. The method described has allowed one to reveal also potentially active sites of hemoglobin amino acid sequence not yet investigated experimentally. The possibility of the natural formation of regulatory oligopeptides from hemoglobin molecules and other food proteins, the formation of an exogenous oligopeptide pool in the gastrointestinal tract, and the conformity of the results to the ideas about the natural continuum of regulatory oligopeptides is considered.
Related Concept Videos
Proteomics
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
The Proteasome Structure
The proteasome is an...
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

