Increased invasion through basement membrane by CXCL7-transfected breast cells
Zhuo Tang1, Minghuan Yu, Fred Miller
1Department of Surgery, Wayne State University, Detroit, MI, USA.
American Journal of Surgery
|October 29, 2008
Summary
CXCL7, a chemokine, promotes breast cancer metastasis by increasing cell invasion. Blocking CXCL7 with an antibody reduced this invasive potential in experimental models.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- CXC chemokines influence breast cancer cell metastasis and extracellular matrix remodeling.
- CXCL7, a heparin-binding chemokine, possesses heparanase activity and binds to the CXCR2 receptor.
Purpose of the Study:
- To investigate the role of CXCL7 and its receptor CXCR2 in breast cancer progression.
- To determine if CXCL7 enhances the invasive potential of breast cancer cells.
Main Methods:
- Utilized isogenic cell lines (MCF10AT and MCF10CA1a.cl1) representing premalignant and malignant states.
- Quantified CXCL7 and CXCR2 expression via RT-PCR and Western blot.
- Assessed cell invasion using matrigel assays and inhibited invasion with a CXCL7 antibody.
Main Results:
- Malignant cells (MCF10CA1a.cl1) showed significantly higher CXCL7 and CXCR2 expression than premalignant cells (MCF10AT).
- Transfection of MCF10AT cells with CXCL7 increased CXCL7 secretion, heparanase activity, and invasiveness.
- Antibody-mediated inhibition of CXCL7 reduced the invasion of CXCL7-transfected cells.
Conclusions:
- Elevated CXCL7 and CXCR2 expression correlates with breast cancer malignancy.
- CXCL7 significantly enhances the invasive capacity of breast cancer cells, mimicking malignant behavior.
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