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Masculinization of XX Drosophila transgenic flies expressing the Ceratitis capitata DoublesexM isoform
Giuseppe Saccone1, Marco Salvemini, Attilio Pane
1Department of Biological Sciences, Genetics and Molecular Biology Section, University of Naples Federico II, Naples, Italy.
Abstract:
The Doublesex (DSX) transcription factor regulates somatic sexual differentiation in Drosophila melanogaster. Female and male isoforms (DSXF and DSXM) are produced due to sex-specific RNA splicing. Here we show that in the distantly related dipteran Ceratitis capitata, the DSXM male-specific isoform is conserved and able to induce masculinization of both somatic and germline tissues when ectopically expressed in XX Drosophila transgenic individuals.
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