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Updated: Jun 28, 2026

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model
Published on: July 1, 2018
[Development of a protocol on pulsed field gel electrophoresis analysis for Streptococcus suis]
Li-Li Wang1, Chang-Yun Ye, Yan-Mei Xu
1State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Objective:
To develop a PFGE protocol for Streptococcus suis.
Methods:
We developed and optimized a PFGE protocol for S. suis, in terms of plug preparation, choice of restriction endonucleases and optimized electrophoresis parameters. By analyzing the genome sequences of S. suis P1/7 with Mapdraw of DNAStar, we found three restriction enzymes, Swa I, Sma I and Apa I, were more suitable than others.
Results:
Analysis of 100 isolates of S. suis including 34 of 35 serotypes identified, 59, 53 and 43 patterns were obtained from Swa I, Sma I and Apa I restriction, respectively. The enzyme Swa I had the greatest power for discrimination ability.
Conclusion:
By optimization of the protocol at various conditions, a rapid, reproducible, economic and practical PFGE method for S. suis was developed.
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