Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An end-to-end hybrid deep-learning approach for single-shot wavefront sensing and correction.

Nature communications·2026
Same author

Can Physical Activity, Sleep Parameters, and Sleep-Wake Patterns Predict Outcome of Combined Chronotherapy in Mood Disorder During Routine Clinical Practice? An Exploratory Study.

Journal of personalized medicine·2026
Same author

Longitudinal Validation of the Artificial Intelligence Algorithm in Home OCT for Age-Related Macular Degeneration-Report 3.

Ophthalmology science·2026
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Home Monitoring for the Management of Age-Related Macular Degeneration: A Review of the Development and Implementation of Digital Health Solutions over a 25-Year Scientific Journey.

Medicina (Kaunas, Lithuania)·2025
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Related Experiment Video

Updated: Jun 28, 2026

Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators
09:23

Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators

Published on: May 30, 2014

Photon-counting optical coherence-domain reflectometry using superconducting single-photon detectors.

Nishant Mohan1, Olga Minaeva, Gregory N Gol'tsman

  • 1Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA. nm82@bu.edu

Optics Express
|October 30, 2008
PubMed
Summary

Superconducting single-photon counting detectors enhance coherence-domain imaging sensitivity. These detectors improve optical coherence-domain reflectometry for biological samples, offering high data acquisition rates.

More Related Videos

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference
07:56

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

Published on: September 5, 2019

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Related Experiment Videos

Last Updated: Jun 28, 2026

Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators
09:23

Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators

Published on: May 30, 2014

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference
07:56

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

Published on: September 5, 2019

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Area of Science:

  • Photonics and Imaging Science
  • Biomedical Optics
  • Quantum Sensing

Background:

  • Coherence-domain imaging techniques are crucial for high-resolution subsurface imaging.
  • Traditional detectors can be limited by noise, affecting sensitivity to weak signals.
  • Single-photon counting detectors offer improved signal-to-noise ratios.

Purpose of the Study:

  • To investigate the utility of single-photon counting detectors for coherence-domain imaging.
  • To experimentally demonstrate the application of superconducting single-photon detectors (SSPDs) in optical coherence-domain reflectometry (OCDR).

Main Methods:

  • Utilizing superconducting single-photon detectors (SSPDs) in an optical coherence-domain reflectometry (OCDR) setup.
  • Evaluating detector performance across the spectral range relevant for biological imaging.
  • Assessing data acquisition rates at high photon flux.

Main Results:

  • SSPDs demonstrate reduced noise operation, enhancing sensitivity for weak light sources and samples.
  • Successful experimental validation of SSPDs for OCDR applications.
  • Broad spectral sensitivity suitable for biological sample imaging.
  • Capability for high data acquisition rates (up to 100 MHz) with sufficient light flux.

Conclusions:

  • Single-photon counting detectors, particularly SSPDs, significantly improve coherence-domain imaging sensitivity.
  • SSPDs are a viable technology for advanced OCDR, enabling detailed imaging of biological tissues.
  • The high sensitivity and acquisition rates of SSPDs open new possibilities in biomedical optics.