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Related Experiment Video

Updated: Jun 28, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

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Published on: February 27, 2026

Thrombin generation before and after multicomponent blood collection.

C Cimenti¹, S Sipurzynski, S Gallistl

¹Medical University of Graz, Department of Paediatrics, Auenbruggerplatz 30, 8036 Graz, Austria. christina.cimenti@meduni-graz.at

Hamostaseologie

|November 5, 2008

Summary

This summary is machine-generated.

Apheresis technology efficiently collects blood components, but donor safety is crucial. This study found that multicomponent apheresis procedures did not significantly alter thrombin generation, indicating good donor hemostatic system safety.

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Area of Science:

Hematology
Transfusion Medicine
Biocompatibility

Background:

Apheresis technology enhances blood component collection efficiency.
Donor safety concerns persist due to blood contact with artificial surfaces.
Assessing the hemostatic impact of apheresis is vital for donor well-being.

Purpose of the Study:

To evaluate the effect of multicomponent apheresis on thrombin generation.
To assess overall coagulation function following apheresis procedures.
To compare thrombin generation between two apheresis systems.

Main Methods:

26 blood donors participated, undergoing apheresis on two different systems (Amicus and Trima Accel).
Platelets and Red Blood Cells (RBCs) were collected in multicomponent procedures.
Thrombin generation was measured using calibrated automated thrombography (CAT) pre-procedure, immediately post-procedure, and 48 hours later.

Main Results:

Calibrated automated thrombography (CAT) data showed only minor, non-significant changes post-apheresis.
No significant differences in thrombin generation parameters were observed at any time point.
Thrombin generation profiles were comparable between the Amicus and Trima Accel apheresis devices.

Conclusions:

Multicomponent apheresis procedures did not induce significant alterations in thrombin generation.
The hemostatic system appears to remain largely unaffected by these apheresis protocols.
Apheresis procedures, as studied, demonstrate a favorable safety profile regarding coagulation function.