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Updated: Jun 28, 2026

One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
Published on: October 23, 2017
Promotion of initiated cells by radiation-induced cell inactivation
W F Heidenreich1, H G Paretzke
1Helmholtz Zentrum München, German Research Center for Environmental Health, Institute for Radiation Protection, Neuherberg, Germany. heidenreich@helmholtz-muenchen.de
Abstract:
Cells on the way to carcinogenesis can have a growth advantage relative to normal cells. It has been hypothesized that a radiation-induced growth advantage of these initiated cells might be induced by an increased cell replacement probability of initiated cells after inactivation of neighboring cells by radiation. Here Monte Carlo simulations extend this hypothesis for larger clones: The effective clonal expansion rate decreases with clone size. This effect is stronger for the two-dimensional than for the three-dimensional situation. The clones are irregular, far from a circular shape. An exposure-rate dependence of the effective clonal expansion rate could come in part from a minimal recovery time of the initiated cells for symmetric cell division.
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