Related Experiment Video

Updated: Jun 28, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

FoxO3a mediates transforming growth factor-beta1-induced apoptosis in FaO rat hepatoma cells

Byung-Chul Kim1

  • 1Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Korea. bckim@kangwon.ac.kr

BMB Reports
|October 31, 2008
PubMed

Insights

Forkhead box O3a (FoxO3a) mediates transforming growth factor-beta1 (TGF-beta1)-induced apoptosis in rat hepatoma cells. This study reveals FoxO3a

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • FoxO3a is a key regulator of apoptosis within the forkhead box O transcription factor family.
  • Transforming growth factor-beta1 (TGF-beta1) is implicated in various cellular processes, including apoptosis.

Purpose of the Study:

  • To investigate the role of FoxO3a in TGF-beta1-induced apoptosis in FaO rat hepatoma cells.
  • To elucidate the signaling pathway involving FoxO3a and TGF-beta1.

Main Methods:

  • Utilized luciferase reporter assays to measure FoxO activity.
  • Employed stable transfection with wild-type FoxO3a and small-interfering RNA (siRNA) for FoxO3a.
  • Assessed apoptosis markers including apoptotic bodies, sub-G1 cell populations, mitochondrial membrane potential, and caspase activation.

Main Results:

  • TGF-beta1 treatment led to time-dependent activation of FoxO3a and increased FoxO reporter activity, sensitive to Akt.
  • Overexpression of FoxO3a enhanced susceptibility to TGF-beta1-induced apoptosis.
  • Knockdown of FoxO3a using siRNA significantly inhibited TGF-beta1-induced caspase activation.

Conclusions:

  • FoxO3a plays a critical role in mediating TGF-beta1-induced apoptosis in FaO rat hepatoma cells.
  • FoxO3a is a crucial component of the TGF-beta1 signaling pathway that triggers apoptosis.

More Related Videos

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity04:20

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Published on: February 9, 2024

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics12:28

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics

Published on: January 11, 2019

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...