Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Atomic Absorption Spectroscopy: Atomization Methods01:25

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Synchronous modulation of fluorescence and oxidase-mimicking activity in a Mn-based MOF nanozyme via post-synthetic metal exchange for Cu<sup>2+</sup> sensing.

Talanta·2026
Same journal

A ratiometric fluorescence sensor based on integrated bimetallic Zr/Eu-MOF for rapid and visual detection of glyphosate.

Talanta·2026
Same journal

Polymeric microneedle patches for rapid DNA extraction and molecular diagnosis of foliar pathogens of mung bean.

Talanta·2026
Same journal

Ce-doped ZnO upconversion nanoparticles for optical thermometry, antibacterial therapy, and cardiac biomarker detection.

Talanta·2026
Same journal

Chiral plasmonic Cu<sub>2-x</sub>S quantum dots enable chirality-dependent fluorescent recognition of tryptophan enantiomers.

Talanta·2026
Same journal

Autocatalytic DNA cascade circuits via split-triggered recombination for ultrasensitive programmable nucleic acid detection.

Talanta·2026

Related Experiment Video

Updated: Jun 28, 2026

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

A borax fusion technique for quantitative X-ray fluorescence analysis.

J H Van Willigen1, H Kruidhof, E A Dahmen

  • 1Twente University of Technology P.O. Box 217 Enschede, Holland.

Talanta
|April 1, 1971
PubMed
Summary

A new borax fusion technique creates glass discs for X-ray analysis. This method is fast, accurate, and easy for new users, comparing well with traditional wet-chemical techniques.

More Related Videos

Expression of Cementitious Pore Solution and the Analysis of Its Chemical Composition and Resistivity Using X-ray Fluorescence
06:27

Expression of Cementitious Pore Solution and the Analysis of Its Chemical Composition and Resistivity Using X-ray Fluorescence

Published on: September 23, 2018

Quantifying X-Ray Fluorescence Data Using MAPS
14:58

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

Related Experiment Videos

Last Updated: Jun 28, 2026

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

Expression of Cementitious Pore Solution and the Analysis of Its Chemical Composition and Resistivity Using X-ray Fluorescence
06:27

Expression of Cementitious Pore Solution and the Analysis of Its Chemical Composition and Resistivity Using X-ray Fluorescence

Published on: September 23, 2018

Quantifying X-Ray Fluorescence Data Using MAPS
14:58

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Quantitative X-ray analysis requires high-quality sample preparation.
  • Traditional methods like wet-chemical analysis can be time-consuming and complex.

Purpose of the Study:

  • To describe a detailed borax fusion technique for preparing glass discs.
  • To highlight critical factors for successful implementation by inexperienced personnel.

Main Methods:

  • Utilizing the non-wetting properties of a Platinum/Gold (Pt/Au) alloy with molten borax.
  • Optimizing flux composition and casting mold design.
  • Developing a straightforward fusion and casting procedure.

Main Results:

  • Successfully produced glass discs suitable for quantitative X-ray analysis.
  • The technique demonstrated ease of use, even for inexperienced workers.
  • Achieved comparable speed and accuracy to established wet-chemical methods.

Conclusions:

  • The described borax fusion technique offers an efficient and reliable method for sample preparation in X-ray analysis.
  • This approach simplifies the process, making it accessible to a wider range of researchers.
  • It presents a viable alternative to traditional analytical techniques.