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Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Related Experiment Video

Updated: Jun 28, 2026

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O₂ with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

Organic analysis by luminescence methods.

J Bartos¹, M Pesez

¹Centre de Recherches Roussel-Uclaf, F.93-Romainville, France.

|February 1, 1972

Summary

This review covers luminescence techniques for quantifying organic compounds in solutions. It proposes a notation for fluorimetric analysis and details methods for both fluorescent and non-fluorescent substances.

Area of Science:

Analytical Chemistry
Spectroscopy

Background:

Luminescence methods offer sensitive detection for organic compounds.
Accurate quantification requires standardized reporting and understanding of influencing factors.

Purpose of the Study:

To review major luminescence techniques for organic compound estimation in solution.
To propose a standardized notation for fluorimetric determinations.
To highlight methods for both naturally fluorescent and non-fluorescent compounds.

Main Methods:

Review of luminescence principles (fluorescence, phosphorescence, chemiluminescence).
Discussion of factors influencing solution fluorescence.
Examples of direct fluorimetric analysis and indirect methods using chemical/enzymatic reactions.

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Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors

Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors

Published on: November 15, 2016

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Last Updated: Jun 28, 2026

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O₂ with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors

Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors

Published on: November 15, 2016

Introduction of functional fluorimetry.

Main Results:

A proposed notation system for fluorimetric determinations.
Illustrative examples of native fluorescence analysis.
Methods for determining non-fluorescent compounds via derivatization to fluorescent species.
Application of enzymatic reactions for chemical quantification.
Demonstration of phosphorimetry and chemiluminescence.

Conclusions:

Luminescence methods, particularly fluorimetry, are versatile for organic compound analysis.
Standardized reporting and functional approaches enhance analytical accuracy.
Chemical and enzymatic transformations expand the scope to non-fluorescent analytes.