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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...

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Related Experiment Video

Updated: Jun 28, 2026

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

Analytical mass spectrometry.

J R Majer1

  • 1Chemistry Department, University of Birmingham, P.O. Box 363, Birmingham 15, U.K.

Talanta
|May 1, 1972
PubMed
Summary

This review covers mass spectrometry fundamentals, instrumentation, and applications. It highlights equipment advancements and their expanding influence on analytical capabilities.

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Mass spectrometry is a powerful analytical technique.
  • Its development has been crucial for scientific advancement.

Purpose of the Study:

  • To review the basic theory of mass spectrometry.
  • To discuss the instrumentation and applications of mass spectrometry.
  • To examine the impact of equipment development on its scope.

Main Methods:

  • Literature review of mass spectrometry.
  • Analysis of historical equipment development.
  • Evaluation of application scope expansion.

Main Results:

  • Mass spectrometry theory provides the foundation for its operation.

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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Analyzing Large Protein Complexes by Structural Mass Spectrometry

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Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
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Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

Published on: September 4, 2010

Related Experiment Videos

Last Updated: Jun 28, 2026

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
08:19

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

Published on: September 4, 2010

  • Instrumentation has evolved significantly, enhancing sensitivity and resolution.
  • New applications have emerged due to technological progress.

    • Conclusions:

    • Mass spectrometry is a versatile technique with a broad range of applications.
    • Continuous equipment development drives innovation and expands its utility.