Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
Gas Chromatography: Overview of Detectors01:13

Gas Chromatography: Overview of Detectors

Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing the effect of obesity-related traits on multiple myeloma using a Mendelian randomisation approach.

Blood cancer journal·2017
Same author

Prediction of outcome in newly diagnosed myeloma: a meta-analysis of the molecular profiles of 1905 trial patients.

Leukemia·2017
Same author

Structure-property relationships in non-epitaxial chalcogenide heterostructures: the role of interface density on charge exchange.

Nanoscale·2016
Same author

Physician response to implementation of genotype-tailored antiplatelet therapy.

Clinical pharmacology and therapeutics·2015
Same author

Genetic factors influencing the risk of multiple myeloma bone disease.

Leukemia·2015
Same author

Extinction during memory reconsolidation blocks recovery of fear in adolescents.

Scientific reports·2015

Related Experiment Video

Updated: Jun 28, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Forced-flow liquid chromatography with a coulometric detector.

D C Johnson1, J Larochelle

  • 1Department of Chemistry, Iowa State University, Ames, Iowa 50010, U.S.A.

Talanta
|October 1, 1973
PubMed
Summary

A novel platinum electrode achieves 100% electrolytic efficiency for electroanalytical applications. This electrode enables precise coulometric determination of copper and iron in liquid chromatography effluents.

More Related Videos

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
08:01

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Related Experiment Videos

Last Updated: Jun 28, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
08:01

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Area of Science:

  • Electrochemistry
  • Analytical Chemistry

Background:

  • Electrochemical methods are crucial for analyzing trace elements.
  • High electrolytic efficiency is desired for accurate quantitative analysis.

Purpose of the Study:

  • To describe a novel tubular platinum electrode.
  • To evaluate its application in coulometric determination of electroactive species.
  • To assess its performance in conjunction with liquid chromatography.

Main Methods:

  • Fabrication of a tubular platinum electrode packed with platinum chips.
  • Application of the electrode for coulometric analysis of effluent from liquid chromatography.
  • Determination of copper (Cu) and iron (Fe) in standard solutions.

Main Results:

  • The electrode demonstrated 100% electrolytic efficiency.
  • Accurate determination of Cu and Fe was achieved.
  • An average relative deviation of less than 2 parts per trillion (ppt) was obtained for samples containing 5 µg of Cu(II) and 5 µg of Fe(III).

Conclusions:

  • The developed platinum electrode is highly efficient for coulometric analysis.
  • It offers excellent precision for determining trace levels of Cu and Fe in complex matrices.
  • The electrode is suitable for online analysis of liquid chromatography effluents.