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Updated: Jun 28, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Coulometric microdetermination of peroxides--I. Hydrogen peroxide
1Department of Analytical Chemistry, Chemical Center, University of Lund, S-220 07 Lund 7, Sweden.
Abstract:
An electroanalytical method, based on controlled-potential coulometry in the iodine-iodide system, has been developed for determination of small amounts of hydrogen peroxide. The sample is added to an electrolyte containing an acid solution of iodide and the iodine, which is produced rapidly, is reduced at a rotating platinum electrode. The number of coulombs consumed in the cell reaction is determined by electronic integration. Samples containing 1 microg-1 mg of hydrogen peroxide were analysed with an accuracy of +/-0.2%. The analysis time ranged from 3 to 7 min.
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