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Related Experiment Video

Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

J Bartos1, M Pesez

  • 1Centre de Recherches Roussel-Uclaf, 93-Romainville, France.

Talanta
|December 1, 1974
PubMed
Summary

A new method uses aromatic aldehydes and thiosemicarbazide to create thiosemicarbazones. These compounds can be quantified using calorimetric determination with 2,3-dichloro-1,4-naphthoquinone.

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Area of Science:

  • Analytical Chemistry
  • Organic Chemistry

Context:

  • Thiosemicarbazones are synthesized from aromatic aldehydes and thiosemicarbazide.
  • The synthesis occurs in an acidic medium.

Purpose:

  • To develop a method for the calorimetric determination of thiosemicarbazones.
  • To quantify trace amounts (micrograms) of synthesized compounds.

Summary:

  • Thiosemicarbazones are extracted into methylene chloride.
  • A colorimetric reaction with 2,3-dichloro-1,4-naphthoquinone enables quantification.

Impact:

  • Provides a sensitive method for analyzing thiosemicarbazone compounds.
  • Applicable to the determination of microgram quantities in various samples.

Related Experiment Videos

Last Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013