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Related Experiment Video

Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

M Pesez1, J Bartos

  • 1Cetre de Recherches Roussel-Uclaf, 93-Romainville, France.

Talanta
|December 1, 1974
PubMed
Summary

This study introduces a colorimetric method for determining carboxylic acids using hydroxamic acid formation and iron(III) development. The technique enables precise quantification of acids, even in ester mixtures.

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Area of Science:

  • Organic Chemistry
  • Analytical Chemistry

Context:

  • Carboxylic acids are fundamental organic compounds with diverse applications.
  • Accurate quantification of carboxylic acids is crucial in various scientific and industrial fields.
  • Existing analytical methods may have limitations in sensitivity or specificity for certain matrices.

Purpose:

  • To develop a sensitive and specific colorimetric method for the determination of carboxylic acids.
  • To utilize the reaction between carboxylic acids and hydroxylamine hydrochloride for quantitative analysis.
  • To establish a reliable method for detecting carboxylic acids in the presence of esters.

Summary:

  • Carboxylic acids react with hydroxylamine hydrochloride and dicyclohexylcarbodiimide to form hydroxamic acids.
  • The resulting hydroxamic acids are complexed with iron(III) for colorimetric detection.
  • This method allows for the quantification of carboxylic acids down to tenths of a milligram, with selectivity over esters, as demonstrated by the determination of acetic acid in butyl acetate at 0.1% concentration.

Impact:

  • Provides a valuable new analytical tool for carboxylic acid quantification.
  • Offers high sensitivity and specificity, suitable for trace analysis.
  • Facilitates quality control and research in chemical and pharmaceutical industries.

Related Experiment Videos

Last Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013