Related Experiment Video
Updated: Jun 28, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Chromatography of aromatic compounds on anion-exchange resins
L M Jahangk1, L Olsson, O Samuelson
1Chalmers University of Technology, Department of Engineering Chemistry, Fack, S-402 20 Göteborg 5, Sweden.
Abstract:
Complex mixtures of aromatic compounds can be rapidly separated on anion-exchangers in the acetate form with acetic acid as eluent and determined automatically by recording the absorbance in the ultraviolet. Carboxylates are separated by ion-exchange. Hydrogen bonds between non-disso-ciated acids and the counter-ions influence the distribution coefficients. Hydrogen-bonding with the resin has a marked effect on the sorption of solutes containing phenolic protons. Intramolecular hydrogen-bonding depresses their sorption. Hydrophobic interactions have a predominant influence with hydrocarbons and with phenolic compounds containing non-polar aliphatic substituents. The relative importance of these interactions increases with a decreased ion-exchange capacity of the resin.
Related Concept Videos
Ion-Exchange Chromatography
Ion Exchange
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Affinity Chromatography
Principles Of Column Chromatography
