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Controlled-Potential Coulometry: Electrolytic Methods

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Related Experiment Video

Updated: Jun 28, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Iodometric determination of ascorbic acid by controlled potential coulometry.

¹Department of Analytical Chemistry, Chemical Center, University of Lund, S-220 07 Lund 7, Sweden.

|December 1, 1975

Summary

This summary is machine-generated.

A new iodometric method using coulometric instrumentation accurately determines ascorbic acid (AA) in pharmaceutical samples within minutes. This method ensures stability and minimizes interference from other sample components.

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Area of Science:

Analytical Chemistry
Electrochemistry
Pharmaceutical Analysis

Background:

Ascorbic acid (Vitamin C) determination is crucial for pharmaceutical quality control.
Existing methods may lack precision or be time-consuming.
Coulometric instrumentation offers a precise electrochemical approach.

Purpose of the Study:

To develop and validate a novel iodometric method for ascorbic acid determination.
To assess the stability of ascorbic acid solutions under various conditions.
To evaluate the method's applicability to pharmaceutical preparations, considering potential interferences.

Main Methods:

Development of an iodometric titration method.
Utilization of previously established coulometric instrumentation.
Stability studies of ascorbic acid solutions.
Analysis of diverse sample matrices, including pharmaceuticals.
Investigation of interfering substances in pharmaceutical samples.

Main Results:

The coulometric iodometric method provides accurate ascorbic acid quantification.
Optimal conditions for ascorbic acid solution stability were established.
The method demonstrated low error (approximately 0.1%).
Analysis time was efficient, ranging from 2 to 6 minutes.
Potential interferences from other sample constituents were evaluated.

Conclusions:

The developed coulometric iodometric method is a reliable and efficient technique for ascorbic acid determination.
The method is suitable for the analysis of pharmaceutical preparations.
Established conditions enhance the reliability of ascorbic acid stability assessments.