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Related Experiment Video

Updated: Jun 28, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Analytical studies using iodine-luminol chemiluminescence.

W M Hardy¹, W R Seitz, D M Hercules

¹Department of Chemistry, University of Georgia, Athens, Georgia 30602, U.S.A.

|May 1, 1977

Summary

Chemiluminescence detection using the iodine-luminol system enables precise measurement of trace substances. This method accurately quanties sulfite, arsenic(III), and sulfur dioxide (SO2) with high reliability.

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Last Updated: Jun 28, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

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Published on: February 16, 2022

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

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Published on: December 25, 2016

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Area of Science:

Analytical Chemistry
Environmental Science

Background:

Chemiluminescence detection offers a sensitive method for analyzing trace substances.
Iodine-based reactions are utilized in various quantitative analyses.

Purpose of the Study:

To develop and validate chemiluminescence-based methods for trace substance quantification.
To assess the precision and accuracy of iodine titrations and rate methods.

Main Methods:

Utilized the iodine-luminol system for chemiluminescence monitoring.
Performed iodine titrations for sulfite and arsenic(III) analysis.
Developed a rate method based on the iodine-penicillin G reaction.
Analyzed a calibrated standard of sulfur dioxide (SO2) in air.

Main Results:

Achieved precision and error better than 1% for sulfite and arsenic(III) titrations.
Analyzed SO2 in air with a precision of +/-1.2% and an error of 0.9%.
Established a linear range from 10(-8) to 10(-7)M for the iodine-penicillin G reaction with +/-9% precision.

Conclusions:

Chemiluminescence detection coupled with iodine chemistry provides a robust platform for trace analysis.
The developed methods demonstrate high accuracy and precision for environmental and pharmaceutical analytes.
The iodine-luminol system is effective for monitoring iodine consumption and related trace substances.