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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Published on: November 15, 2013
[Not Available].
1Service Central de Microanalyse du Centre National de la Recherche Scientifique, 2 rue Henry Dunant, 94320 Thiais, France.
Talanta
|August 1, 1977
Summary
This study details a wet chemical method for analyzing trace metals like aluminum, cobalt, tin, and iron using spectrophotometry. The process effectively quanties these elements in small samples via specific complexation reactions.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Inorganic Chemistry
Context:
- Accurate quantification of trace metals is crucial in various scientific disciplines.
- Traditional methods may require larger sample sizes or more complex procedures.
- Development of efficient analytical techniques for elemental determination is ongoing.
Purpose:
- To establish a reliable wet chemical method for the spectrophotometric determination of aluminum, cobalt, tin, and iron.
- To outline the specific complexation reactions utilized for each metal.
- To enable the analysis of small sample masses (up to 5 mg).
Summary:
- A wet digestion process using a Kjeldahl flask is employed to prepare samples.
- Aluminum is quantified using Alizarin Red S complexation.
- Cobalt(II) is determined via tiron complexation.
- Tin(IV) is analyzed using phenylfluorone complexation.
- Iron(III) is quantified using tiron complexation.
- All determinations are performed spectrophotometrically.
Impact:
- Provides a method for accurate trace metal analysis with minimal sample consumption.
- Applicable to various matrices where these elements need to be quantified.
- Contributes to the field of analytical chemistry by offering a specific spectrophotometric protocol.
