Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...

Atomic Absorption Spectroscopy: Lab

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

Microbial Bioremediation of Uranium

Microbial Bioremediation of Uranium

Microorganisms play a critical role in the transformation and immobilization of uranium in contaminated environments through four main pathways: bioreduction, biosorption, bioaccumulation, and biomineralization. These mechanisms reduce uranium’s toxicity and prevent its migration through groundwater systems, offering sustainable approaches for in situ bioremediation.Bioreduction of UraniumBioreduction is driven by anaerobic bacteria such as certain strains of Geobacter and Shewanella, which use...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A phase II, randomized, open-label study to evaluate low-dose pembrolizumab plus chemotherapy versus chemotherapy as neoadjuvant therapy for localized triple-negative breast cancer (TNBC) (PLANeT trial-Pembrolizumab Low dose in Addition to NACT in TNBC).

Annals of oncology : official journal of the European Society for Medical Oncology·2025

Same author

Fabrication and analysis of enhanced thermal stability and high-sensitivity turnaround point long-period fiber grating.

Applied optics·2022

Same author

A Comparative Study of Enriched Culture Media and Selective Culture in the Diagnosis of Gonorrhoea.

Indian journal of dermatology and venereology·2017

Same author

Caregiver burden in Alzheimer-type dementia and psychosis: A comparative study from India.

Asian journal of psychiatry·2017

Same author

Flow and thermal characteristics of high Reynolds number (2800-17,000) dye cell: simulation and experiment.

Applied optics·2015

Same author

Predominance of modern Mycobacterium tuberculosis isolates in North India.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2014

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Separation of Uranium and Thorium for 230Th-U Dating of Submarine Hydrothermal Sulfides

Separation of Uranium and Thorium for ²³⁰Th-U Dating of Submarine Hydrothermal Sulfides

Published on: May 20, 2019

Sensitive spectrophotometric method for trace amounts of uranium.

O Prakash¹, S Kumar, S P Mushran

¹Chemical Laboratories, University of Allahabad, Allahabad, India.

|December 1, 1979

Summary

This study introduces a sensitive spectrophotometric method for uranium determination using Pyrogallol Red and cetyltrimethylammonium bromide. The developed method enhances sensitivity and allows for accurate uranium quantification in various samples.

More Related Videos

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Published on: June 21, 2015

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Published on: January 30, 2018

Related Experiment Videos

Last Updated: Jun 28, 2026

Separation of Uranium and Thorium for 230Th-U Dating of Submarine Hydrothermal Sulfides

Separation of Uranium and Thorium for ²³⁰Th-U Dating of Submarine Hydrothermal Sulfides

Published on: May 20, 2019

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Published on: June 21, 2015

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Published on: January 30, 2018

Area of Science:

Analytical Chemistry
Inorganic Chemistry

Background:

Accurate determination of uranium is crucial for environmental monitoring and nuclear industry applications.
Existing spectrophotometric methods for uranium often lack sufficient sensitivity or require complex procedures.

Purpose of the Study:

To develop a highly sensitive spectrophotometric method for uranium (U) determination.
To investigate the complexation reaction between uranium, Pyrogallol Red (PGR), and cetyltrimethylammonium bromide (CTAB).

Main Methods:

Spectrophotometric analysis of uranium using Pyrogallol Red (PGR) as a chromogenic agent.
Inclusion of cetyltrimethylammonium bromide (CTAB) to enhance the sensitivity of the color reaction.
Characterization of the ternary complex formed, including its composition and optimal reaction conditions (pH 5.6).

Main Results:

A sensitive blue ternary complex of uranium with PGR and CTAB was formed, with maximum absorbance at 580 nm.
The method demonstrated adherence to Beer's law over a uranium concentration range of 0.24–19.0 ppm.
High molar absorptivity (3.3 x 10^4 L.mol^-1.cm^-1) was achieved at 620 nm, indicating excellent sensitivity.

Conclusions:

The developed spectrophotometric method offers a sensitive and reliable approach for uranium quantification.
The presence of CTAB significantly enhances the sensitivity of the uranium-PGR reaction.
The study also proposes a method for evaluating the stability constants of such ternary complexes.