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Related Experiment Video

Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

A Verbert1, G Nowogrocki, M Wozniak

  • 1Ecole Nationale Supérieure de Chimie de Lille, Université de Lille I, B.P. 40, 59650 Villeneuve d'Ascq, France.

Talanta
|May 1, 1980
PubMed
Summary
This summary is machine-generated.

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This study precisely determines proton concentration and calculates dissociation constants for dibasic acids using differential spectrophotometry and acid-base indicators. The method accurately measured pK values for protonated O-phosphoserine.

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Biochemistry

Context:

  • Determining acid dissociation constants is crucial for understanding chemical and biological systems.
  • Traditional methods for measuring these constants can be complex and time-consuming.
  • Protonated O-phosphoserine is a biologically relevant molecule with two dissociation steps.

Purpose:

  • To develop and validate a precise spectrophotometric method for determining proton concentration.
  • To calculate the first two dissociation constants (pK values) of protonated O-phosphoserine.
  • To demonstrate the utility of differential spectrophotometry with acid-base indicators for this purpose.

Summary:

  • A novel method utilizing differential spectrophotometry and an acid-base indicator precisely quantifies proton concentration from moderately strong dibasic acid dissociation.

Related Experiment Videos

Last Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

  • Dissociation constants are subsequently calculated via linear regression analysis.
  • The method was successfully applied to determine the pK(1) = 0.72 and pK(2) = 2.14 values for protonated O-phosphoserine in aqueous solution at 25°C and 0.1M KNO(3).

    • Impact:

    • Provides a more accurate and efficient method for determining acid dissociation constants.
    • Facilitates the study of biologically relevant molecules like O-phosphoserine.
    • Offers a valuable tool for researchers in analytical, physical, and biological chemistry.