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Updated: Jun 28, 2026

A Computer-assisted Multi-electrode Patch-clamp System
Published on: October 18, 2013
Microprocessor-controlled system for automatic acquisition of potentiometric data and their non-linear least-squares
H Gampp1, M Maeder, A D Zuberbühler
1Institute of Inorganic Chemistry, University of Basel, CH-4056 Basel, Switzerland.
Abstract:
A microprocessor-controlled potentiometric titration apparatus for equilibrium studies is described. The microprocessor controls the stepwise addition of reagent, monitors the pH until it becomes constant and stores the constant value. The data are recorded on magnetic tape by a cassette recorder with an RS232 input-output interface. A non-linear least-squares program based on Marquardt's modification of the Newton-Gauss method is discussed and its performance in the calculation of equilibrium constants is exemplified. An HP 9821 desk-top computer accepts the data from the magnetic tape recorder. In addition to a fully automatic fitting procedure, the program allows manual adjustment of the parameters. Three examples are discussed with regard to performance and reproducibility.
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