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Related Experiment Video

Updated: Jun 28, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

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Published on: April 11, 2014

Determination of ascorbic acid with ferricyanide.

N Rukmini¹, V S Kavitha, K Devendra Vijaya

¹Department of Chemistry, Andhra University, Waltair 530003, India.

|May 1, 1981

Summary

This summary is machine-generated.

This study presents a reliable titration method to quantify ascorbic acid (vitamin C) using potassium ferricyanide. The assay is effective for commercial products and unaffected by common interfering organic substances.

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Area of Science:

Analytical Chemistry
Biochemistry

Background:

Ascorbic acid (vitamin C) is a vital nutrient with numerous health benefits.
Accurate quantification of ascorbic acid is crucial for quality control of supplements and food products.

Purpose of the Study:

To establish a robust titration method for determining ascorbic acid content.
To evaluate the suitability of various indicators for this titration.
To assess the method's applicability to real-world samples and its specificity.

Main Methods:

Titration of ascorbic acid with potassium ferricyanide.
Utilized acidic media including sulfuric, phosphoric, and hydrochloric acids.
Tested twelve different indicators, such as ferroin and various thiazine and triphenylmethane dyes.

Main Results:

The titration method proved effective in acidic media.
Twelve indicators were identified as suitable for the assay.
The method demonstrated successful application in assaying commercial vitamin C products.
No interference was observed from common organic substances found in preparations and plant tissues.

Conclusions:

Potassium ferricyanide titration is a viable method for ascorbic acid determination.
The method is accurate and specific, suitable for quality control of vitamin C products.
The use of diverse indicators provides flexibility in the analytical procedure.