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Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
10:50

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Published on: April 10, 2016

Combined assay and determination of bioactivity of polyene antibiotics by flow nephelometry

A E Beezer1, B Z Chowdhry

  • 1Department of Chemistry, Chelsea College, University of London, Manresa Road, London SW3 6LX, England.

Talanta
|September 1, 1981
PubMed

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Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...