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Updated: Jun 28, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Photometric determination of nitrite
1Departments of Chemistry, College of Science and College of Education, Mosul University, Mosul, Iraq.
Abstract:
A new sensitive colour reaction for nitrite determination is presented. The method is based on the reaction of nitrite with p-aminobenzoic acid to form a diazonium ion, which is coupled with 8-hydroxyquinoline, in alkaline medium, to form a reddish-orange water-soluble azo dye. Diazotization and coupling are very fast and control of temperature is unnecessary. Moreover, the colour is stable and its intensity is in direct proportion to nitrite concentration over a wide range.
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