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Updated: Jun 28, 2026

High-Throughput Measurement and Classification of Organic P in Environmental Samples
Published on: June 8, 2011
Differential pulse polarographic determination of orthophosphate in aqueous media
S C Hight1, F Bet-Pera, B Jaselskis
1Loyola University of Chicago, Chicago, Illinois 60626, U.S.A.
Abstract:
The determination of orthophosphate in aqueous media by differential pulse polarography is described. It is based on determination of the molybdenum in 12-phosphomolybdic acid. High sensitivity is achieved by measuring the polarographic wave due to the catalytic reduction of perchlorate or nitrate in the presence of molybdenum(VI). The method is suitable for samples as small as 3.5 ml which contain as little as 9 ng of phosphorus per ml. The average relative deviation is 3.0% at the 0.045 mg/l. phosphorus level and 1.6% at the 1.2 mg/l. level. Results for the analysis of EPA quality-control water and real surface-water samples are reported.
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