Related Experiment Video
Updated: Jun 28, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Semi-xylenol orange and its purification by high-pressure liquid chromatography
F Smedes1, L G Decnop-Weever, N T Uyen
1Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 166, Amsterdam, The Netherlands.
Abstract:
By Mannich condensation of o-Cresol Red, iminodiacetic acid and formaldehyde, Semi-Xylenol Orange (SXO) has been prepared in a 10-hr batch-procedure with a yield of about 30%. From the crude product SXO has been isolated by reversed-phase HPLC with perchloric acid-acetone mixtures as the mobile phase and C(18)-bonded silica as the stationary phase. The SXO fraction was freed from accompanying perchloric acid by a second separation on the same column, with water as eluent. After elution with acetone, the SXO was crystallized by evaporation.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Principles Of Column Chromatography
