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Updated: Jun 28, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
Published on: September 13, 2014
Polarographic study of simple and mixed-ligand complex formation
1Facultad de Ciencias Exactas de la Universidad National de La Plata, 1900-La Plata, Argentina.
Abstract:
Theoretical, experimental and statistical aspects of the polarographic method applied to simple and mixed-ligand complex formation have been studied. Some misunderstandings and erroneous interpretations have been clarified according to experimental results obtained with several systems. A criterion for establishing the number of complex species present in a simple system is proposed. For the first time the polarographic method has been applied to a mixed-ligand complex system where fourteen species co-exist.
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