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Updated: Jun 28, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Gel speciation studies-I The intrinsic dissociation constant of weakly acidic cation-exchange gels
1Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14214, U.S.A.
Abstract:
The arid-dissociation properties of three weakly acidic cation-exchange gels, CM-Sephadex C-50-120, Biogel CM-2 and CM-Biogel A, have been studied. Each of the gels was equilibrated in sodium polystyrene sulphonate (NaPSS) solutions at three different concentration levels (0.1, 0.01 and 0.001M). The volume of the gels was measured as a function of a (their degree of neutralization), and C(p) (the NaPSS concentration); pH and pNa were also measured at each alpha-value. Intrinsic pK values of 3.25 and 4.55 have been found for CM-Sephadex C-50-120 and Biogel CM-2 respectively.
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